Application
Western blot analysis of karyopherin β3 expression in non-transfected 293T: (A), human karyopherin β3 transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | karyopherin β3 Antibody (A-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of karyopherin β3 of human origin |
| Modification Notes | Protein transport across the nucleus is a selective, multi-step process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Two cytosolic factors centrally involved in the recognition and docking process are the karyopherin å1 and karyopherin ∫1 subunits. Karyopherin å1 functions in the recognition and targeting of substrates destined for nuclear import, while karyopherin ∫1 serves as an adapter, tethering the karyopherin å1/substrate complex to docking proteins on the nuclear envelope, termed nucleoporins. Karyopherin å2 has been shown to complex with Epstein-Barr virus nuclear antigen 1 (EBNA-1). Certain RNA-binding proteins are imported to the nucleus by karyopherin ∫2, and karyopherin ∫3 appears to be involved in the import of some ribosomal proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIFELISA |
| recommended for detection of karyopherin β3 of mouse, rat and human origin by WB, IF and ELISA non cross-reactive with other karyopherin family members also reactive with additional species, including equine, canine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of karyopherin &beta 3 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of karyopherin β3 of human origin |
| Entrez Gene | 3843 |
|---|
Application
Western blot analysis of karyopherin β3 expression in non-transfected 293T: (A), human karyopherin β3 transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of karyopherin β3 of mouse, rat and human origin by WB, IF and ELISA non cross-reactive with other karyopherin family members also reactive with additional species, including equine, canine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
