Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of Fis1 expression in A-431 (A) and HeLa (B) whole cell lysates.| Product Name | Fis1 Antibody (FL-152) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-152 representing full length Fis1 of human origin |
| Modification Notes | Fis1 localizes to the outer mitochondrial membrane and, along with dynamin-related protein (Drp1), participates in mitochondrial fission. Fission and fusion mechanisms regulate mitochondrial morphology within the cell. Fission frequency is determined by the level of Fis1 molecules at the mitochondrial surface. Fis1 contains a C-terminal domain, which is required for mitochondrial localization, and an N-terminal domain, which is necessary for mitochondrial fission. Fragmentation of the mitochondrial network by Fis1 leads to cytochrome c release and apoptosis. The mitochondrial fission mechanisms may be involved in positively and negatively regulating apoptosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Fis1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-152 representing full length Fis1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-152 representing full length Fis1 of human origin |
| Entrez Gene | 51024 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of Fis1 expression in A-431 (A) and HeLa (B) whole cell lysates.| Application Notes | recommended for detection of Fis1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
