Application
Western blot analysis of FBPase expression in non-transfected: (A) and human FBPase transfected: (B) 293T whole cell lysates.Application
Western blot analysis of liver FBPase expression in non-transfected: (A) and mouse liver FBPase transfected: (B) 293T whole cell lysates.| Product Name | FBPase Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of liver FBPase of human origin |
| Modification Notes | Fructose-1,6-bisphosphatase (FBPase) mediates the key reaction of carbohydrate metabolism. It catalyzes the splitting of fructose-1,6-bisphosphate into fructose 6-phosphate and inorganic phosphate. FBPase is encoded by two genes, FBP1 and FBP2, which express the liver and muscle isoforms, respectively. FBPase appears to be present in all living organisms and is regulated by AMP inhibition in most species. Inhibition of FBPase by AMP affects the turnover of bound substrate and not its affinity for substrate. The liver FBPase isoform is composed of four identical subunits. Mutations in the FBP1 gene are inherited as an autosomal recessive disorder that leads to a deficiency of FBPase, which is associated with hypoglycemia and metabolic acidosis. Muscle FBPase is located on both sides of the z-line. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of liver and muscle FBPase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of liver FBPase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of liver FBPase of human origin |
| Entrez Gene | 2203 |
|---|
Application
Western blot analysis of FBPase expression in non-transfected: (A) and human FBPase transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of liver FBPase expression in non-transfected: (A) and mouse liver FBPase transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of liver and muscle FBPase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
