Application
Western blot analysis of Cytokeratin 5 expression in SW480 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human uterine cervix tissue showing cytoplasmic staining of squamous epithelial cells.| Product Name | Cytokeratin 5 Antibody (H-40) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-40 mapping at the N-terminus of Cytokeratin 5 of human origin |
| Modification Notes | Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. Cytokeratin 5 is expressed in normal basal cells. Mutations of the cytokeratin 5 gene (KRT5) have been shown to result in the autosomal dominant disorder epidermolysis bullosa (EB). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Cytokeratin 5 and, to a lesser extent, Cytokeratin 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-40 mapping at the N-terminus of Cytokeratin 5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-40 mapping at the N-terminus of Cytokeratin 5 of human origin |
| Entrez Gene | 3852 |
|---|
Application
Western blot analysis of Cytokeratin 5 expression in SW480 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human uterine cervix tissue showing cytoplasmic staining of squamous epithelial cells.| Application Notes | recommended for detection of Cytokeratin 5 and, to a lesser extent, Cytokeratin 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
