Application
Western blot analysis of Ankyrin-1 expression in non-transfected: (A) and human Ankyrin-1 transfected: (B) 293T whole cell lysates.| Product Name | Ankyrin-1 Antibody (H-125) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 820-952 mapping within an internal region of Ankyrin-1 isoform 9 of human origin |
| Modification Notes | Members of the Ankyrin family of proteins mediate the attachment of integral membrane proteins to the cytoskeleton. ANK1, ANK2 and ANK3 genes encode for the proteins in this family, Ankyrin-1 (also designated Ankyrin R), Ankyrin B and Ankyrin G, respectively. The proteins are structured similarly each composed of an N-terminal domain with multiple ankyrin repeats, a highly conserved central spectrin binding domain, and C-terminal regulatory domains which are susceptible to the most variance. Ankyrin-1 is a membrane protein that links the cytoskeleton to the plasma membrane in erythrocytes, cardiac and skeletal muscle, and brain. It is expressed as many isoforms, including a full length protein and several shorter isoforms. Ankyrin-1 has also been found to be defective in patients with hereditary spherocytosis (HS), a common hemolytic anemia. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ankyrin-1 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with Ankyrin-1-2, Ankyrin-1-3, Ankyrin-1-4, Ankyrin-1-5, Ankyrin-1-7, Ankyrin-1-8, Ankyrin-1-9, Ankyrin-1-10, Ankyrin-1-11 Ankyrin-1-12, Ankyrin-1-13, Ankyrin-1-Er16, Ankyrin-Mu18, Ankyrin-1-Mu19, Ankyrin-1-Mu20, or Ankyrin-1-Br21 also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 820-952 mapping within an internal region of Ankyrin-1 isoform 9 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 820-952 mapping within an internal region of Ankyrin-1 isoform 9 of human origin |
| Entrez Gene | 286 |
|---|
Application
Western blot analysis of Ankyrin-1 expression in non-transfected: (A) and human Ankyrin-1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Ankyrin-1 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with Ankyrin-1-2, Ankyrin-1-3, Ankyrin-1-4, Ankyrin-1-5, Ankyrin-1-7, Ankyrin-1-8, Ankyrin-1-9, Ankyrin-1-10, Ankyrin-1-11 Ankyrin-1-12, Ankyrin-1-13, Ankyrin-1-Er16, Ankyrin-Mu18, Ankyrin-1-Mu19, Ankyrin-1-Mu20, or Ankyrin-1-Br21 also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
