Application
Western blot analysis of AdoMetDC expression in non-transfected: (A) and mouse AdoMetDC transfected: (B) 293T whole cell lysates.Application
Western blot analysis of AdoMetDC expression in non-transfected: (A) and mouse AdoMetDC transfected: (B) 293T whole cell lysates.| Product Name | AdoMetDC Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 7-209 of AdoMetDC of human origin |
| Modification Notes | Polyamines are compounds that have two or more primary amino groups and are important to cellular processes, such as cellular growth, proliferation and tumor promotion. AdoMetDC (adenosylmethionine decarboxylase 1), also known as S-adenosylmethionine decarboxylase proenzyme (SAMDC) or AMD1, is a 334 amino acid protein which is an important intermediate enzyme in polyamine biosynthesis pathways. Using a pyruvoyl group as a cofactor, AdoMetDC catalyzes the conversion of S-adenosyl-L-methionine to (5-deoxy-5-adenosyl)(3-aminopropyl)-methylsulfonium salt and carbon dioxide. AdoMetDC is synthesized as an inactive proenzyme that undergoes self-maturation to form two non-identical subunits designated å and ∫. Active AdoMetDC forms a heterotetramer of two å chains and two ∫ chains. Both AdoMetDC proenzyme processing and mature AdoMetDC catalytic activity are stimulated by putrescine, while catalytic activity is inhibited by iodoacetic acid. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of AdoMetDC of mouse, rat and human origin by WB, IP, IF and ELISA also recommended for detection of Amd2 of mouse origin also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 7-209 of AdoMetDC of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 7-209 of AdoMetDC of human origin |
| Entrez Gene | 262 |
|---|
Application
Western blot analysis of AdoMetDC expression in non-transfected: (A) and mouse AdoMetDC transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of AdoMetDC expression in non-transfected: (A) and mouse AdoMetDC transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of AdoMetDC of mouse, rat and human origin by WB, IP, IF and ELISA also recommended for detection of Amd2 of mouse origin also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
