Application
Western blot analysis of LDH-A expression in non-transfected 293T: (A), mouse LDH-A transfected 293T: (B) and SK-N-SH (C) whole cell lysates.Application
Western blot analysis of LDH expression in 293T whole cell lysate (A) and mouse testis tissue extract (B).Application
Western blot analysis of LDH-C expression in non-transfected: (A) and human LDH-C transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immuno?fluorescence staining of methanol-fixed HeLa cells showing cytoplasmic local?ization.Application
Western blot analysis of LDH expression in SK-N-SH (A), SJRH30 (B) and LADMAC (C) whole cell lysates.| Product Name | LDH Antibody (H-160) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 173-332 mapping at the C-terminus of LDH-A of human origin |
| Modification Notes | The lactate dehydrogenase family (LDH) catalyzes the final step of anaerobic glycolysis, the conversion of L-lactate and NAD to pyruvate and NADH. The LDH family consists of three members, LDH-A, LDH-B and LDH-C, all of which form tetramers consisting four subunits. However, each family member displays a specific tissue distribution pattern with LDH-A and LDH-B predominant in several tissues, specifically LDH-A in muscle and LDH-B in heart, while LDH-C expression is confined to the testis and sperm. LDHs function as powerful markers for germ cell tumors. The genes encoding human LDH-A and LDH-C map to chromosome 11, while the human LDH-B gene maps to chromosome 12. Deficiency in the LDH-A gene is linked to exertional myoglobinuria. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of LDH-A, B, C and LDH-A-like 6A and 6B of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 173-332 mapping at the C-terminus of LDH-A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 173-332 mapping at the C-terminus of LDH-A of human origin |
| Entrez Gene | 3939 |
|---|
Application
Western blot analysis of LDH-A expression in non-transfected 293T: (A), mouse LDH-A transfected 293T: (B) and SK-N-SH (C) whole cell lysates.
Application
Western blot analysis of LDH expression in 293T whole cell lysate (A) and mouse testis tissue extract (B).
Application
Western blot analysis of LDH-C expression in non-transfected: (A) and human LDH-C transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immuno?fluorescence staining of methanol-fixed HeLa cells showing cytoplasmic local?ization.
Application
Western blot analysis of LDH expression in SK-N-SH (A), SJRH30 (B) and LADMAC (C) whole cell lysates.| Application Notes | recommended for detection of LDH-A, B, C and LDH-A-like 6A and 6B of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
