| Product Name | INSIG-1 Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 31-100 mapping near the N-terminus of INSIG-1 of human origin |
| Modification Notes | INSIG-1 and INSIG-2 play distinct roles in a negative-feedback mechanism for cholesterol synthesis. INSIG-1 is highly expressed in liver and fibroblast cell lines. INSIG-1 localizes to the endoplasmic reticulum (ER) and binds the sterol-sensing domain of SREBP cleavage-activating protein (SCAP). Sterol induces INSIG-1 binding to SCAP. INSIG-2, another ER protein, binds SCAP in a sterol-regulated manner. Thus, INSIG-1 and INSIG-2 block the export of SCAP from the ER and ultimately inhibit cholesterol synthesis by preventing the proteolytic processing of SREBPs by Golgi enzymes. INSIG-1 is encoded by the Insulin-induced gene (INSIG-1). INSIG-1 gene expression is suppressed by oxysterols and restored following the introduction of the hypocholester-olemic agent LY295427. The negative feedback mechanism is absent in mutant CHO cells with a point mutation in one SCAP allele within the sterol-sensing domain. The mutant cells constitutively cleave SREBP in the presence of sterols. The critical role of INSIG-1 and INSIG-2 in cholesterol metabolism may be exploited as a therapeutic effect for hypercholesterolemia. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of INSIG-1 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 31-100 mapping near the N-terminus of INSIG-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 31-100 mapping near the N-terminus of INSIG-1 of human origin |
| Entrez Gene | 3638 |
|---|
| Application Notes | recommended for detection of INSIG-1 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
