Application
Western blot analysis of PTBP-2 expression in rat skeletal muscle tissue extract.Application
Western blot analysis of PTBP-2 expression in non-transfected: (A) and human PTBP-2 transfected: (B) 293T whole cell lysates.| Product Name | PTBP-2 Antibody (H-40) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 138-177 mapping within an internal region of PTBP-2 of human origin |
| Modification Notes | PTBP-2 (polypyrimidine tract binding protein 2), also known as PTB or nPTB (neural polypyrimidine tract-binding protein), is a member of the polypyrimidine tract binding family of proteins. Predominantly expressed in brain, but also found in heart and skeletal muscle, PTBP-2 localizes to the nucleus and contains four RRM (RNA recognition motif) domains. PTBP-2 functions as an RNA-binding protein associated in a complex that is involved in the regulation of exon splicing and the stabilization of mRNAs in the cytoplasm. Six isoforms exist for PTBP-2 due to alternative splicing events. Isoforms 1 and 2 (also known as nPTB1 and nPTB2/PTBPLP-L, respectively) are neuronal-specific. Isoforms 3 and 4 (also known as nPTB3/PTBPLP-L and nPTB4, respectively) are found in non-neuronal tissues, as are isoforms 5 and 6 (also known as nPTB5/nPTB7/PTBPLP-S and nPTB6/nPTB8/PTBPLP-S, respectively). The existence of various isoforms may function to modulate the RNA-binding properties of PTBP-2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PTBP-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 138-177 mapping within an internal region of PTBP-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 138-177 mapping within an internal region of PTBP-2 of human origin |
| Entrez Gene | 58155 |
|---|
Application
Western blot analysis of PTBP-2 expression in rat skeletal muscle tissue extract.
Application
Western blot analysis of PTBP-2 expression in non-transfected: (A) and human PTBP-2 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of PTBP-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
