Application
Western blot analysis of GRAF expression in non-transfected (A) and human GRAF transfected (B) 293 whole cell lysates.Application
Western blot analysis of GRAF expression in mouse brain tissue lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast cancer tissue showing cytoplasmic localization.| Product Name | GRAF Antibody (Y-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of GRAF of mouse and human origin by WB, IP, IF, IHC(P), ELISA |
| Modification Notes | Cellular signaling by G-proteins is down-regulated by GTPase-activating proteins (GAPs), which increase the rate of GTP hydroylsis. The GTPase regulator associated with focal adhesion kinase (GRAF) has GAP activity toward Rho A and Cdc42, but not Rac1. GRAF is ubiquitously expressed with high levels in heart and brain. Expression of GRAF causes clearing of stress fibers and formation of long actin based filopodial-like extensions. Fusion of MLL with GRAF, MLL/GRAF, is included in a rare genetic subgroup of acute myeloid leukemia (AML) cases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of GRAF of mouse and human origin by WB IP IF IHC(P) ELISA |
|---|---|
| Tissue Specificity | recommended for detection of GRAF of mouse and human origin by WB, IP, IF, IHC(P), ELISA |
| Entrez Gene | 23092 |
|---|
Application
Western blot analysis of GRAF expression in non-transfected (A) and human GRAF transfected (B) 293 whole cell lysates.
Application
Western blot analysis of GRAF expression in mouse brain tissue lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast cancer tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
