Application
Western blot analysis of Lingo-1 expression in non-transfected (A) and human Lingo-1 transfected (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human brain tissue showing cytoplasmic localization.| Product Name | Lingo-1 Antibody (F-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Lingo-1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Lingo-1 is a 614-amino acid protein that plays an important role in the negative regulation of myelination by oligodendrocytes in the central nervous system (CNS). Lingo-1 is a nervous system-specific transmembrane protein that interacts with NgR1 and p75 to make up a receptor complex that binds to Nogo, a protein that inhibits axonal regeneration. Reduction of Lingo-1 activity downregulates RhoA (a protein related to cytoskeleton regulation) activity, promotes oligodendrocyte differentiation, and increases axonal myelination in neuronal tissues. Conversely, overexpression of Lingo-1 activates RhoA and inhibits oligodendrocyte differentiation and myelination. Lingo-1 up-regulation may be a characteristic of activity-induced neural plasticity responses. Lingo-1 may be a critical deterrent of myelin and nerve fiber repair in multiple sclerosis, an inflammatory disease that causes gradual destruction of myelin in the CNS. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Lingo-1 of human origin by WB, IP, IF, IHC(P) and ELISA: WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Lingo-1 of human origin by WB IP IF IHC(P) and ELISA |
|---|
| Entrez Gene | 84894 |
|---|
Application
Western blot analysis of Lingo-1 expression in non-transfected (A) and human Lingo-1 transfected (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human brain tissue showing cytoplasmic localization.| Application Notes | recommended for detection of Lingo-1 of human origin by WB, IP, IF, IHC(P) and ELISA: WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
