Application
Western blot analysis of ERp57 expression in non-transfected 293T: (A), human ERp57 transfected 293T: (B) and Daudi (C) whole cell lysates.Application
Western blot analysis of ERp57 expression in non-transfected 293T: (A), mouse ERp57 transfected 293T: (B) and Daudi (C) whole cell lysates.Application
Western blot analysis of ERp57 expression in non-transfected 293T: (A), mouse ERp57 transfected 293T: (B) and Daudi (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine pancreas at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of ERp57 expression in NRK whole cell lysate.Application
Western blot analysis of ERp57 expression in mouse liver tissue extract (A) and NRK (B) and Caki-1 (C) whole cell lysates.| Product Name | ERp57 Antibody (H-220) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 108-207 mapping within an internal region of ERp57 of human origin |
| Modification Notes | Mammals defend themselves against intracellular pathogens through presentation of cytoplasmically-derived short pathogenic peptides to the cell surface of cytotoxic T lymphocytes, which subsequently leads to cytotoxic events with respect to the affected cell. Antigen presentation is mediated by major histocompatibility complex (MHC) class I molecules, which bind and coordinate short pathogenic peptides. MHC class I molecules assemble in the endoplasmic reticulum with chaperones before binding to the transporter associated with antigen processing (TAP). ERp57, also designated GRP57, GRP58, ERp60 and ERp61, is a component of the MHC class I pathway that appears to interact with MHC class I molecules before they associate with TAP. The human ERp57 gene maps to chromosome 15q15 and encodes a 505 amino acid protein. ERp57 has two Trp-Cys-Gly-His-Cys-Lys motifs completely conserved among the mammals. ERp57 may act as a protease, a protein disulfide isomerase, a phospholipase or a combination of these. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of ERp57 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 108-207 mapping within an internal region of ERp57 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 108-207 mapping within an internal region of ERp57 of human origin |
| Entrez Gene | 2923 |
|---|
Application
Western blot analysis of ERp57 expression in non-transfected 293T: (A), human ERp57 transfected 293T: (B) and Daudi (C) whole cell lysates.
Application
Western blot analysis of ERp57 expression in non-transfected 293T: (A), mouse ERp57 transfected 293T: (B) and Daudi (C) whole cell lysates.
Application
Western blot analysis of ERp57 expression in non-transfected 293T: (A), mouse ERp57 transfected 293T: (B) and Daudi (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine pancreas at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of ERp57 expression in NRK whole cell lysate.
Application
Western blot analysis of ERp57 expression in mouse liver tissue extract (A) and NRK (B) and Caki-1 (C) whole cell lysates.| Application Notes | recommended for detection of ERp57 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
