Application
Nuclear Ki67 in mouse mammary gland(60X microscopic magnification in oil). Dilution 1:50 in dilution buffer (0.05% BSA in PBS) Blocking: 0.1% BSA in PBS at room temp. Kindly provided by Dr. Albert J. Fornace Jr., Georgetown University.Application
Western blot analysis of Ki-67 expression in MCF7 nuclear extract.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human normal tonsil showing nuclear staining. Data kindly provided by D. Mason.| Product Name | Ki-67 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 2641-2940 of Ki-67 of human origin |
| Modification Notes | Ki-67 is a nuclear protein that is expressed in proliferating cells and may be required for maintaining cell proliferation. Ki-67 has been used as a marker for cell proliferation of solid tumors and some hematological malignancies. A correlation has been demonstrated between Ki-67 index and the histopathological grade of neoplasms. Assessment of Ki-67 expression in renal and ureter tumors shows a correlation between tumor proliferation and disease progression, thus making it possible to differentiate high-risk patients. Ki-67 expression may also prove to be important for distinguishing between malignant and benign peripheral nerve sheath tumors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Ki-67 of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 2641-2940 of Ki-67 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 2641-2940 of Ki-67 of human origin |
| Entrez Gene | 4288 |
|---|
Application
Nuclear Ki67 in mouse mammary gland(60X microscopic magnification in oil). Dilution 1:50 in dilution buffer (0.05% BSA in PBS) Blocking: 0.1% BSA in PBS at room temp. Kindly provided by Dr. Albert J. Fornace Jr., Georgetown University.
Application
Western blot analysis of Ki-67 expression in MCF7 nuclear extract.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human normal tonsil showing nuclear staining. Data kindly provided by D. Mason.| Application Notes | recommended for detection of Ki-67 of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
