Application
Western blot analysis of RNase HII-A expression in Jurkat whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic localization.| Product Name | RNase HII-A Antibody (V-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of RNase HII-A of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | The RNase HII complex is an endonuclease that degrades RNA found in RNA:DNA duplexes and is composed of one catalytic subunit and two non-catalytic subunits. RNase HII-A, also called RNASEH2A (Ribonuclease H2 subunit A), RNASEHI, AGS4 or RNHIA, is the 299 amino acid catalytic subunit of RNase HII. Localized to the nucleus, RNase HII-A mediates the removal of Okazaki fragment RNA primers that are present on the lagging strand during DNA replication. RNase HII-A catalyzes the endonucleolytic cleavage of RNA to a 5’-phosphomonoester and is able to bind magnesium or manganese as cofactors. Defects in the gene encoding RNase HII-A are the cause of Aicardi-Goutieres syndrome type 4 (AGS4), an autosomal recessive encephalopathy characterized by cerebral atrophy, leukodystrophy, intracranial calcifications and chronic cerebrospinal fluid (CSF) lymphocytosis. Patients affected by AGS4 have severe neurological dysfunctions and often die in early childhood. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of RNase HII-A of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of RNase HII-A of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 10535 |
|---|
Application
Western blot analysis of RNase HII-A expression in Jurkat whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
