Application
Western blot analysis of DDX30 expression in non-transfected: (A) and mouse DDX30 transfected: (B) 293T whole cell lysates.| Product Name | DDX30 Antibody (K-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of DDX30 of human origin by WB, IP and ELISA |
| Modification Notes | Characterized by the conserved motif Asp-Glu-Ala-Asp, DEAD box proteins are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure. Specifically, DEAD box proteins are involved in translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX30, DDX35 and DDX36 each contain 1 helicase ATP-binding domain and 1 helicase C-terminal domain. DDX30 (DEAH box protein 30)is a 1,194 amino acid protein that forms a complex with TFAM and SSBP1 in the mitochondria, suggesting a role for DDX30 in mtDNA replication. There are two isoforms of DDX30 that exist as a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of DDX30 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of DDX30 of human origin by WB, IP and ELISA |
| Entrez Gene | 22907 |
|---|
Application
Western blot analysis of DDX30 expression in non-transfected: (A) and mouse DDX30 transfected: (B) 293T whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
