Application
Western blot analysis of LRRC50 expression in 721 B whole cell lysate.| Product Name | LRRC50 Antibody (X-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of LRRC50 of mouse, rat and human origin by WB, IP and ELISA |
| Modification Notes | The leucine-rich (LRR) repeat is a 20-30 amino acid motif that forms a hydrophobic å/∫ horseshoe fold, allowing it to accommodate several leucine residues within a tightly packed core. All LRR repeats contain a variable segment and a highly conserved segment, the latter of which accounts for 11 or 12 residues of the entire LRR motif. LRRC50 (leucine rich repeat containing 50) is a 725 amino acid protein that localizes to the cellular projection, as well as to the spindle and the cytoplasm, and contains six LRR repeats. Existing as multiple alternatively spliced isoforms, LRRC50 functions as a cilum-specific protein that is required for the maintenance of ciliary architecture and is also involved in the regulation of both Actin-based brush border and microtubule-based cilia microvilli. Due to its association with microvilli, LRRC50 may be involved in the pathogenesis of polycystic kidney disease. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of LRRC50 of mouse rat and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of LRRC50 of mouse, rat and human origin by WB, IP and ELISA |
| Entrez Gene | 123872 |
|---|
Application
Western blot analysis of LRRC50 expression in 721 B whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
