Application
Western blot analysis of Raly expression in Jurkat whole cell lysate.Application
Western blot analysis of Raly expression in HeLa whole cell lysate.| Product Name | Raly Antibody (Q-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Raly of human origin by WB, IIP, F, IHC(P) and ELISA |
| Modification Notes | Raly, also known as hnRNP associated with lethal yellow homolog, Autoantigen p542 or Heterogeneous nuclear ribonucleoprotein C-like 2 (hnRNPCL2), is a 306 amino acid protein belonging to the RRM HNRPC family. Localized to the nucleus, Raly is thought to be an RNA-binding protein involved in pre-mRNA splicing. Phosphorylated upon DNA damage, Raly has been found to be a subunit of the spliceosome C complex. Raly is also an autoantigen that is cross-reactive with an Epstein-Barr virus protein. Raly is expressed at higher levels in pancreas, liver, skeletal muscle, lung, brain, heart and kidney with lower levels found in placenta. Produced by alternative splicing, Raly is expressed as two isoforms. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Raly of human origin by WB IIP F IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Raly of human origin by WB, IIP, F, IHC(P) and ELISA |
| Entrez Gene | 22913 |
|---|
Application
Western blot analysis of Raly expression in Jurkat whole cell lysate.
Application
Western blot analysis of Raly expression in HeLa whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
