Application
Western blot analysis of Rab 25 expression in non-transfected: (A) and human Rab 25 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Rab 25 expression in non-transfected: (A) and human Rab 25 transfected: (B) 293T whole cell lysates.| Product Name | Rab 25 Antibody (H-51) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 163-213 mapping at the C-terminus of Rab 25 of human origin |
| Modification Notes | The Ras-related superfamily of guanine nucleotide binding proteins includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies. Increasing data suggests an important role for Rab proteins in either endocytosis or in biosynthetic protein transport. The process of transporting newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves the movement of carrier vesicles and requires Rab protein function. Rab proteins are also an integral part of endocytic pathways. Rab 25, also known as CATX-8 or Rab 11c, is a member of the Rab family of proteins that is exclusively expressed in epithelial cells and participates in apical vesicle trafficking. Rab 25 is overexpressed in ovarian and breast cancer cells and has been associated with metastasis and tumor aggressiveness. The forced expression of Rab 25 in ovarian and breast cancer cells increases cell proliferation in vivo. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Rab 25 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 163-213 mapping at the C-terminus of Rab 25 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 163-213 mapping at the C-terminus of Rab 25 of human origin |
| Entrez Gene | 57111 |
|---|
Application
Western blot analysis of Rab 25 expression in non-transfected: (A) and human Rab 25 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Rab 25 expression in non-transfected: (A) and human Rab 25 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Rab 25 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
