Application
Western blot analysis of ALDH4A1 expression in non-transfected: (A) and mouse ALDH4A1 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human muscle tissue showing cytoplasmic localization.Application
Western blot analysis of ALDH4A1 expression in human fetal liver tissue extract.| Product Name | ALDH4A1 Antibody (F-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of ALDH4A1 of mouse, human and zebrafish origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Modification Notes | Aldehyde dehydrogenases (ALDHs) mediate NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde, lipid peroxidation and metabolism of corticosteroids, biogenic amines and neurotransmitters. ALDH4A1 (aldehyde dehydrogenase 4 family member A1), also known as P5CD (Delta-1-pyrroline-5-carboxylate dehydrogenase), P5CDh, P5CDhL, P5CDhS or ALDH4, is a major enzyme involved in the proline degradation pathway. Localizing to the mitochondrial matrix, ALDH4A1 catalyzes the conversion of Delta-1-pyrroline-5-carboxylate (P5C) to glutamate. A mutation in the gene encoding ALDH4A1 results in HPII (hyperprolinemia type II), a disease characterized by an excess of P5C and proline that is associated with mental retardation and seizures. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of ALDH4A1 of mouse human and zebrafish origin by WB IP IF IHC(P) and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of ALDH4A1 of mouse, human and zebrafish origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 8659 |
|---|
Application
Western blot analysis of ALDH4A1 expression in non-transfected: (A) and mouse ALDH4A1 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human muscle tissue showing cytoplasmic localization.
Application
Western blot analysis of ALDH4A1 expression in human fetal liver tissue extract.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
