Application
Western blot analysis of Parkin phosphorylation in 293 cells transfected with wild type Parkin (A) and Ser 378 mutated (non-phosphorylated) Parkin (B).| Product Name | p-Parkin Antibody (Ser 378) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Parkin is a zinc-finger protein that is related to ubiquitin at the amino terminus. The wild type Parkin gene, which maps to human chromosome 6q25.2-27, encodes a 465 amino acid full-length protein that is expressed as multiple isoforms. Mutations in the Parkin gene are responsible for autosomal recessive juvenile Parkinson’s disease and commonly involve deletions of exons 3-5. In humans, Parkin is expressed in a subset of cells of the basal ganglia, midbrain, cerebellum and cerebral cortex, and is subject to alternative splicing in different tissues. Parkin expression is also high in the brainstem of mice, with the majority of immunopositive cells being neurons. The Parkin gene has been identified in a diverse group of organisms including mammals, birds, frog and fruit flies, suggesting that analogous functional roles of the Parkin protein may have been highly conserved during the course of evolution. Human Parkin is phosphorylated on Ser 378 at the C-terminus by CK-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 378 phosphorylated Parkin of human origin by WB |
|---|---|
| Tissue Specificity | recommended for detection of Ser 378 phosphorylated Parkin of human origin by WB |
| Entrez Gene | 5071 |
|---|
Application
Western blot analysis of Parkin phosphorylation in 293 cells transfected with wild type Parkin (A) and Ser 378 mutated (non-phosphorylated) Parkin (B).| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
