Application
Western blot analysis of PDH-E1α expression in Hep G2 whole cell lysate.Application
Western blot analysis of PDH-E1α expression in rat brain tissue extract.| Product Name | PDH-E1α Antibody (E-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial matrix enzyme complex that functions as the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The E1 enzyme of the PDH complex is made up of a heterotetramer of two å and two ∫ subunits. The E1-å subunit (PDH-E1å) contains the E1 active site and plays a key role in the function of the PDH complex. The PDH complex is regulated by phosphorylation and dephosphorylation of PDH-E1å. The gene encoding for PDH-E1å maps to chromosome Xp22-q24, and a 20-bp deletion in the last exon of this gene is sufficient to cause PDH deficiency, which causes a broad range of symptoms including the development of seizures, mental retardation and spasticity, as well as intermittent episodes of lactic acidosis associated with cerebellar ataxia. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of PDH-E1&alpha of mouse rat and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of PDH-E1α of mouse, rat and human origin by WB, IP and ELISA |
| Entrez Gene | 5160 |
|---|
Application
Western blot analysis of PDH-E1α expression in Hep G2 whole cell lysate.
Application
Western blot analysis of PDH-E1α expression in rat brain tissue extract.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
