Application
Western blot analysis of PIPK II γ expression in mouse brain tissue extract.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Product Name | PIPK II γ Antibody (C-16) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of PIPK II γ of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Phosphatidylinositol phosphate kinases (PIPKs) synthesize phosphatidylinositol-4,5-bisphosphate, which regulates various processes including cell proliferation, survival, membrane trafficking and cytoskeletal organization. The PIPK family is divded into three different classes, designated type I, type II and type III, each of which contain an activation loop, which determines their enzymatic specificity and subcellular targeting. The type I PIPKs (PIPK I) consist of PIPK I å, ∫ and ©, while the type II PIPKs (PIPK II) consist of PIPK II å and ∫, both of which exhibit high levels of expression in the brain. Type III PIPK (designated PIP5K III) localizes to the endosome membrane where it participates in endosome-related membrane trafficking and, like other PIPK proteins, generates phosphatidylinositol-4,5-bisphosphate via ATP-dependent phosphorylation. Due to their ability to regulate phosphatidylinositol-4,5-bisphosphate production, the PIPK proteins are essential messengers for signal transduction pathways throughout the body. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of PIPK II &gamma of mouse and human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of PIPK II γ of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 79837 |
|---|
Application
Western blot analysis of PIPK II γ expression in mouse brain tissue extract.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
