Application
Western blot analysis of PHPT1 expression in non-transfected: (A) and human PHPT1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PHPT1 expression in 293 showing nontransfected (A) and transfected (B) whole cell lysates.| Product Name | PHPT1 Antibody (N-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | PHPT1 (Phosphohistidine phosphatase 1), also known as 14 kDa phosphohistidine phosphatase, is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. First identified in human adrenal gland, PHPT1 is highly expressed in skeletal muscle and heart, with lower expression in liver, pancreas and kidney. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. PHPT1 is highly conserved among species, suggesting that it serves an essential functional role. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of PHPT1 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of PHPT1 of human origin by WB , IP and ELISA |
| Entrez Gene | 29085 |
|---|
Application
Western blot analysis of PHPT1 expression in non-transfected: (A) and human PHPT1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PHPT1 expression in 293 showing nontransfected (A) and transfected (B) whole cell lysates.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
