Application
Western blot analysis of eIF4E2 expression in non-transfected 293T: (A), mouse eIF4E2 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of eIF4E2 expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic staining.| Product Name | eIF4E2 Antibody (E-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against eIF4E2 of human origin |
| Modification Notes | The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation factor 4E family (eIF4E) is comprised of three proteins that are involved in the early initiation of protein synthesis. eIF4E2 (eukaryotic translation initiation factor 4E family member 2), also known as 4EHP, IF4e, 4E-LP or EIF4EL3, is a ubiquitously expressed 245 amino acid protein. During early translation events, eIF4E2 recognizes and binds the 7-methylguanosine-containing mRNA cap (a cotranscriptionally added structure that conveys mRNA stability and allows for efficient RNA processing), thus initiating the unwinding of mRNA secondary structures and facilitating mRNA-ribosome binding. eIF4E2 competes with eIF4E (member 1) for cap binding and, upon modification by the ubiquitin-like protein ISG15 (Interferon-induced 15 kDa protein), exhibits increased mRNA cap affinity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of eIF4E2 of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against eIF4E2 of human origin |
|---|---|
| Tissue Specificity | raised against eIF4E2 of human origin |
| Entrez Gene | 9470 |
|---|
Application
Western blot analysis of eIF4E2 expression in non-transfected 293T: (A), mouse eIF4E2 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of eIF4E2 expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic staining.| Application Notes | recommended for detection of eIF4E2 of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
