Application
Blots were probed with p-Stat3 (Ser 727): (A) and p-Stat3 (Ser 727): preincubated with its cognate non-phosphorylated peptide (B) and p-Stat3 (Ser 727): preincubated with its cognate phosphorylated peptide (C).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.| Product Name | p-Stat3 Antibody (Ser 727) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 727 phosphorylated Stat3 of mouse, rat and human origin by WB, IP, IF and IHC(P) |
| Modification | Phosphoralated |
| Modification Notes | Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-a and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3∫ appears to be activated by both while Stat3å is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| See 1 citations for p-Stat3Antibody (Ser 727): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 727 phosphorylated Stat3 of mouse rat and human origin by WB IP IF and IHC(P) |
|---|---|
| Tissue Specificity | recommended for detection of Ser 727 phosphorylated Stat3 of mouse, rat and human origin by WB, IP, IF and IHC(P) |
| Entrez Gene | 6774 |
|---|
Application
Blots were probed with p-Stat3 (Ser 727): (A) and p-Stat3 (Ser 727): preincubated with its cognate non-phosphorylated peptide (B) and p-Stat3 (Ser 727): preincubated with its cognate phosphorylated peptide (C).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.| Application Notes | See 1 citations for p-Stat3Antibody (Ser 727): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
