Application
Antibodies tested include p-connexin 43 (Ser 368): (A,B) and connexin 43 (C-20)-R: (C,D).Application
Western blot analysis of connexin 43 phosphorylation in non-transfected: (A), untreated mouse connexin 43 transfected: (B) and lambda protein phosphatase treated mouse connexin 43 transfected: (C) 293T whole cell lysates.Application
Western blot analysis of connexin 43 phosphorylation in non-transfected: (A) and mouse connexin 43 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of phosphorylated connexin 43 expression in untreated (A,B,C) and PMA-treated (D,E,F) HeLa (A,D), K-562 (B,E) and 293 (C,F) whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.| Product Name | p-connexin 43 Antibody (Ser 368) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 368 of connexin 43 of human origin |
| Modification | Phosphoralated |
| Modification Notes | The connexins are a group of gap junction proteins which form a hexamer to compose a connexon. Clusters of connexons form a gap junction through which low molecular weight proteins may diffuse from cell to cell. Several mammalian cells with malignant phenotypes exhibit decreased connexin expression and gap junction communication. There is a decrease in gap junctional communication in Src transformed cells. The decreased communication appears to be associated with tyrosine phosphorylation of Connexin 43. Activated c-Src phosphorylates the C-terminal tail of Connexin 43 on residue Tyr 265, resulting in a stable interaction between both proteins leading to inhibition of gap junctional communication. In addition to tyrosine phosphorylation, Connexin 43 has also been shown to be phosphorylated on serine in the absence of Src kinases and on both serine and tyrosine in cells expressing Src kinases, such as pp60v-Src and/or c-Src. In human vascular endothelial cells, Connexin 43 is postranslationally modified during mitosis. Mitosis-specific phosphorylation of Connexin 43 correlates with the transient loss of gap junction intercellular communication and redistribution of Connexin 43. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Ser 368 phosphorylated connexin 43 of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 368 of connexin 43 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 368 of connexin 43 of human origin |
| Entrez Gene | 2697 |
|---|
Application
Antibodies tested include p-connexin 43 (Ser 368): (A,B) and connexin 43 (C-20)-R: (C,D).
Application
Western blot analysis of connexin 43 phosphorylation in non-transfected: (A), untreated mouse connexin 43 transfected: (B) and lambda protein phosphatase treated mouse connexin 43 transfected: (C) 293T whole cell lysates.
Application
Western blot analysis of connexin 43 phosphorylation in non-transfected: (A) and mouse connexin 43 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of phosphorylated connexin 43 expression in untreated (A,B,C) and PMA-treated (D,E,F) HeLa (A,D), K-562 (B,E) and 293 (C,F) whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.| Application Notes | recommended for detection of Ser 368 phosphorylated connexin 43 of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
