Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.Application
Western blot analysis of phosphorylated CREB-1 expression in untreated (A) and UV-treated (B) 293 whole cell lysates.| Product Name | p-CREB-1 Antibody (Ser 129) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 129 of CREB-1 of human origin |
| Modification | Phosphoralated |
| Modification Notes | Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA (1,2). The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding (3). Although CREB can bind to DNA in an unphosphorylated state, it cannot activate transcription (4). Phosphorylation of CREB on Ser133 by protein kinase A facilitates its interaction with the CREB-binding protein (CBP) and activates the basal transcription complex (5,6). CREB functions in neoglucogenesis through interactions with the nuclear coactivator PGC-1 (7). CREB may play a role in the pathogenesis of type II diabetes and dilated cardiomyopathy (7,8). The gene encoding CREB-1 maps to human chromosome 2q32.3-q34 (9). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Ser 129 phosphorylated CREB-1 of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 129 of CREB-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 129 of CREB-1 of human origin |
| Entrez Gene | 1385 |
|---|
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.
Application
Western blot analysis of phosphorylated CREB-1 expression in untreated (A) and UV-treated (B) 293 whole cell lysates.| Application Notes | recommended for detection of Ser 129 phosphorylated CREB-1 of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
