Application
Antibodies tested include p-IRS-1 (Ser 312): (A, B, C) and IRS-1 (A-19): (D, E, F).Application
Western blot analysis of IRS-1 phosphorylation in non-transfected: (A), untreated human IRS-1 transfected: (B) and lambda protein phosphatase treated human IRS-1 transfected: (C) 293T whole cell lysates.Application
Blots were probed with p-IRS-1 (Ser 312): preincubated with cognate phosphorylated peptide (A) and p-IRS-1 (Ser 312): (B).| Product Name | p-IRS-1 Antibody (Ser 312) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 312 of IRS-1 of human origin |
| Modification | Phosphoralated |
| Modification Notes | Insulin receptor substrate-1 (IRS-1) is a substrate of the insulin receptor that undergoes phosphorylation in response to insulin, IGF-1 and IL-4. Tyrosine (Tyr) phosphorylation of IRS-1 mediates insulin-stimulated responses, while Serine (Ser)/Threonine (Thr) phosphorylation of IRS-1 can either enhance or negate insulin effects. Tyrosines 465, 612, 632, 662, 941 and 989 of IRS-1 resemble YXXM motifs that upon phosphorylation are predicted to bind SH2 domains in the p85 regulatory subunit of PI3K, resulting in activation of p110 catalytic subunit. SHP-2 binding to IRS-1 can occur upon phosphorylation at Tyr 1179 and Tyr 1229. GRB2 binding can occur upon phsophoryl-ation at Tyr 896. Rodent Ser 99 and Thr 502 of IRS-1 are casein kinase II-dependent phosphorylation sites. There is an increase in Ser 636 phosphoryl-ation of IRS-1 in primary skeletal muscle cells from patients with type 2 diabetes. IGF-I and anisomycin treatment converge downstream onto FRAP and PKCd to induce IRS-1 Ser 312 phosphorylation. Insulin resistance in the aorta of hypertensive rats is associated with elevated IRS-1 phosphorylation at Ser 307 and increased SAPK/JNK activation. IRS-1 contains three putative binding sites for 14-3-3 protein at Ser 270, Ser 374 and Ser 641 that are capable of phosphorylation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| recommended for detection of Ser 312 phosphorylated IRS-1 of human origin and correspondingly phosphorylated Ser 307 of mouse and rat origin by WB and IP: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 312 of IRS-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 312 of IRS-1 of human origin |
| Entrez Gene | 3667 |
|---|
Application
Antibodies tested include p-IRS-1 (Ser 312): (A, B, C) and IRS-1 (A-19): (D, E, F).
Application
Western blot analysis of IRS-1 phosphorylation in non-transfected: (A), untreated human IRS-1 transfected: (B) and lambda protein phosphatase treated human IRS-1 transfected: (C) 293T whole cell lysates.
Application
Blots were probed with p-IRS-1 (Ser 312): preincubated with cognate phosphorylated peptide (A) and p-IRS-1 (Ser 312): (B).| Application Notes | recommended for detection of Ser 312 phosphorylated IRS-1 of human origin and correspondingly phosphorylated Ser 307 of mouse and rat origin by WB and IP: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
