Application
Western blot analysis of DHODH expression in THP-1 (A) and SK-BR-3 (B) whole cell lysates and human kidney tissue extract (C).| Product Name | DHODH Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of DHODH of human origin |
| Modification Notes | DHODH (dihydroorotate dehydrogenase), also known as DHOdehase, is a 395 amino acid mitochondrial protein located on the outer surface of the inner mitochondrial membrane. It catalyzes the fourth enzymatic step in de novo pyrimidine biosynthesis. De novo pyrimidine synthesis is a critical metabolic pathway for nucleic acid synthesis and is a target for various cancer chemotherapy agents. Additionally, DHODH is functionally connected to the respiratory chain, delivering electrons to ubiquinone. DHODH contains a bipartite signal at the N-terminus that regulates passage into the mitochondrial inner membrane. The inhibition of COX (cytochrome c oxidase) by nitric oxide (NO) indirectly inhibits DHODH activity. The inhibition of DHODH has an immunosuppressive and an antiproliferative effect on diseases such as rheumatoid arthritis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DHODH of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of DHODH of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of DHODH of human origin |
| Entrez Gene | 1723 |
|---|
Application
Western blot analysis of DHODH expression in THP-1 (A) and SK-BR-3 (B) whole cell lysates and human kidney tissue extract (C).| Application Notes | recommended for detection of DHODH of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
