Application
Immunofluores-cence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of 20S Proteasome α5 expression in non-transfected: (A) and mouse 20S Proteasome α5 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of 20S Proteasome α5 expression in non-transfected: (A) and mouse 20S Proteasome α5 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of 20S Proteasome α5 expression in non-transfected: (A) and mouse 20S Proteasome α5 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of 20S Proteasomeα 5 expression in non-transfected 293T: (A), human 20S Proteasomeα 5 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of 20S Proteasome α5 expression in HeLa nuclear extract (A) and HeLa (B), Caki-1 (C), JAR (D), JEG-3 (E) and SW480 (F) whole cell lysates.| Product Name | 20S Proteasome α5 Antibody (H-53) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 41-93 mapping near the N-terminus of 20S Proteasome α5 of human origin |
| Modification Notes | The proteasome represents a large protein complex that exists inside all eukaryotes and archaea, and in some bacteria. The main function of proteasomes is to degrade unnecessary or damaged proteins by proteolysis. The most common form of the proteasome, known as the 26S Proteasome, contains one 20S Proteasome core particle structure and two 19S regulatory caps. The 20S Proteasome core is hollow and forms an enclosed cavity, where proteins are degraded, as well as openings at the two ends to allow the target protein to enter. The 20S Proteasome core particle contains many subunits, depending on the organism. All of the subunits fall into one of two types: å subunits, which are structural, serve as docking domains for the regulatory particles and exterior gates blocking unregulated access to the interior cavity; or ∫ subunits, which are predominantly catalytic. The outer two rings in the proteasome consist of seven å subunits each, and the inner two rings each consist of seven ∫ subunits. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of 20S Proteasome α5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 41-93 mapping near the N-terminus of 20S Proteasome &alpha 5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 41-93 mapping near the N-terminus of 20S Proteasome α5 of human origin |
| Entrez Gene | 5686 |
|---|
Application
Immunofluores-cence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of 20S Proteasome α5 expression in non-transfected: (A) and mouse 20S Proteasome α5 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of 20S Proteasome α5 expression in non-transfected: (A) and mouse 20S Proteasome α5 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of 20S Proteasome α5 expression in non-transfected: (A) and mouse 20S Proteasome α5 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of 20S Proteasomeα 5 expression in non-transfected 293T: (A), human 20S Proteasomeα 5 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of 20S Proteasome α5 expression in HeLa nuclear extract (A) and HeLa (B), Caki-1 (C), JAR (D), JEG-3 (E) and SW480 (F) whole cell lysates.| Application Notes | recommended for detection of 20S Proteasome α5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
