Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human seminal vesicle tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of MetAP-2 expression in non-transfected: (A) and human MetAP-2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of MetAP-2 expression in non-transfected 293T: (A), human MetAP-2 transfected 293T: (B) and A-431 (C) whole cell lysates.Application
Western blot analysis of MetAP-2 expression in non-transfected 293T: (A), human MetAP-2 transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | MetAP-2 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 159-458 mapping near the C-terminus of MetAP-2 of human origin |
| Modification Notes | Methionine aminopeptidases (MetAP), also designated peptidase M proteins, are members of the M24 family of proteins. Both MetAP-1 and MetAP-2 release N-terminal amino acids, usually methionine, from nascent peptides and arylamines. Eukaryotes contain both MetAP-1 and MetAP-2, whereas prokaryotes possess only the MetAP-1 enzyme. MetAP-1 and MetAP-2 control cell proliferation in mammalian cells. MetAP-2 is highly conserved between human and Saccharomyces cerevisiae. Neurofibromin (NF1) regulates MetAp-2 and increased expression of MetAP-2 correlates with several forms of cancer. Inhibitors of MetAP-2 are potential targets in cancer therapeutics, particularly in NF1-associated tumor proliferation. Chemotherapeutic drugs such as ovalicin and fumagillin bind to the active site of and inhibit MetAp-2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MetAP-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 159-458 mapping near the C-terminus of MetAP-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 159-458 mapping near the C-terminus of MetAP-2 of human origin |
| Entrez Gene | 10988 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human seminal vesicle tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of MetAP-2 expression in non-transfected: (A) and human MetAP-2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of MetAP-2 expression in non-transfected 293T: (A), human MetAP-2 transfected 293T: (B) and A-431 (C) whole cell lysates.
Application
Western blot analysis of MetAP-2 expression in non-transfected 293T: (A), human MetAP-2 transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of MetAP-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
