Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of FcRn expression in non-transfected: (A) and mouse FcRn transfected: (B) 293T whole cell lysates.| Product Name | FcRn Antibody (M-255) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 22-276 mapping within an N-terminal extracellular domain of FcRn of mouse origin |
| Modification Notes | FcRN present in the intestinal epithelium of neonatal mice and rats mediates the selective uptake of immunoglobulin G (IgG) in mothers' milk, thereby helping newborn animals to acquire passive immunity. FcRn (also designated FCGRT, Brambell receptor, FcRn å chain, IgG Gc receptor and neonatal Fc-receptor) is comprised of a heavy chain and ∫-2-Microglobulin. FcRn heavy chain shows approximately 35% amino acid identity to an MHC class I molecule. FcRN localizes in endosomes of vascular endothelial cells and selectively recycles IgG to the cell surface, thus protecting IgG from lysosomal catabolism. This protection mechanism is a major constituent for ensuring IgG are the longest lived of all plasma proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of FcRn of mouse, rat and, to a lesser extent, human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 22-276 mapping within an N-terminal extracellular domain of FcRn of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 22-276 mapping within an N-terminal extracellular domain of FcRn of mouse origin |
| Entrez Gene | 2217 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of FcRn expression in non-transfected: (A) and mouse FcRn transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of FcRn of mouse, rat and, to a lesser extent, human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
