Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of GPx-3 expression in HeLa whole cell lysate.Application
Western blot analysis of GPx-3 expression in Hep G2 whole cell lysate.Application
Western blot analysis of GPx-6 expression in non-transfected 293T: (A), human GPx-6 transfected 293T: (B) and Hep G2 (C) whole cell lysates.| Product Name | GPx-3 Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 21-70 mapping near the N-terminus of GPx-3 of human origin |
| Modification Notes | Glutathione peroxidase (GPx) enzymes are generally selenium-containing tetrameric glycoproteins that help prevent lipid peroxidation of cell membranes. GPx enzymes reduce lipid hydroperoxides to alcohols, and reduce free hydrogen peroxide to water. GPx members are among the few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. There are eight GPx homologs (GPx-1–8). GPx-1 plays an important role in the antioxidant defense of the vascular wall and neural cells in response to oxidative stress. GPx-2 is the major isoform in the lungs and its basal or inducible expression is dependent on Nrf2. GPx-3 is under regulation by hypoxic stress and the expression and deficiency of GPx-3 is associated with cardiovascular disease and stroke. GPx-5 is selenium-independent; it is bound to the acrosome of sperm, where it may protect sperm from premature acrosome reaction in the epididymis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GPx-3 and, to a lesser extent, GPx-5 and GPX-6 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 21-70 mapping near the N-terminus of GPx-3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 21-70 mapping near the N-terminus of GPx-3 of human origin |
| Entrez Gene | 2878 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of GPx-3 expression in HeLa whole cell lysate.
Application
Western blot analysis of GPx-3 expression in Hep G2 whole cell lysate.
Application
Western blot analysis of GPx-6 expression in non-transfected 293T: (A), human GPx-6 transfected 293T: (B) and Hep G2 (C) whole cell lysates.| Application Notes | recommended for detection of GPx-3 and, to a lesser extent, GPx-5 and GPX-6 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
