Application
Western blot analysis of ZNF499 expression in HeLa (A) and A-431 (B) nuclear extracts and human kidney tissue extract (C).Application
Western blot analysis of ZNF499 expression in Jurkat whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing nuclear localization.| Product Name | ZNF499 Antibody (Z-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of ZNF499 of human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Modification Notes | The BTB (Broad-Complex, Tramtrack and Bric a brac) domain, also known as the POZ (POxvirus and Zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. ZNF499 (zinc finger protein 499), also known as ZBTB45 (zinc finger and BTB domain containing 45), is a 511 amino acid nuclear protein that contains one BTB domain and four C2H2-type zinc fingers and is thought to be involved in transcriptional regulation events. The gene encoding ZNF499 maps to human chromosome 19, which consists of over 63 million bases, houses approximately 1,400 genes and is recognized for having the greatest gene density of the human chromosomes. It is the genetic home for a number of immunoglobulin (Ig) superfamily members, including the killer cell and leukocyte Ig-like receptors, a number of ICAMs, the CEACAM and PSG family and Fc receptors (FcRs). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of ZNF499 of human origin by WB IP IF IHC(P) and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of ZNF499 of human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 84878 |
|---|
Application
Western blot analysis of ZNF499 expression in HeLa (A) and A-431 (B) nuclear extracts and human kidney tissue extract (C).
Application
Western blot analysis of ZNF499 expression in Jurkat whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing nuclear localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
