Application
Western blot analysis of ZNF589 expression in Hep G2 whole cell lysate.| Product Name | ZNF589 Antibody (A-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of ZNF589 of human origin by WB, IP and ELISA |
| Modification Notes | Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krüppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. ZNF589 (zinc finger protein 589), also known as SZF1 or stem cell zinc finger protein 1, is a 364 amino acid protein that plays a role in the differentiation of hematopoietic stem cells, and may at as a repressor of DNA binding-dependent transcription. ZNF589 belongs to the krueppel C2H2-type zinc-finger protein family, localizes to nucleus, and contains four C2H2-type zinc fingers and one KRAB domain. As a result of alternative splicing, three ZNF589 isoforms exist; while the expression of ZNF589 isoform 1 has not been fully characterized, isoform 2 (also known as SZF1-2) has been found to be ubiquitously expressed and isoform 3 (also known as SZF1-1) is only found in CD34+ cells. The gene encoding ZNF589 maps to human chromosome 3p21.31. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of ZNF589 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of ZNF589 of human origin by WB, IP and ELISA |
| Entrez Gene | 51385 |
|---|
Application
Western blot analysis of ZNF589 expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
