Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of casein kinase Iα expression in HeLa whole cell lysate.Application
Western blot analysis of casein kinase Iα expression in K-562 whole cell lysate.Application
Western blot analysis of CSNK1A1L expression in non-transfected: (A) and human CSNK1A1L transfected: (B) 293T whole cell lysates.Application
Western blot analysis of casein kinase Iα expression in non-transfected: (A) and mouse casein kinase Iα transfected: (B) 293T whole cell lysates.Application
Western blot analysis of casein kinase Iα expression in non-transfected 293T: (A), mouse casein kinase Iα transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | casein kinase Iα Antibody (H-57) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 281-337 mapping at the C-terminus of casein kinase Iα of human origin |
| Modification Notes | Casein kinase I (also designated CKI) and casein kinase II (CKII) compose a family of serine/threonine protein kinases which are present in all eukaryotes examined to date. Casein kinase I family members, which include casein kinase Iå, I©, I∂ and Ié, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair, membrane trafficking, circadian rhythm, cell cycle progression, chromosome segregation, apoptosis and cellular differentiation. Casein kinase I isoform alpha-like (CSNK1A1L) is a 337 amino acid protein that shares a high degree of sequence similarity with the alpha isoform of casein kinase 1. CSNK1A1L resides in the cytoplasm and participates in the Wnt signaling pathway. By utilizing ATP within its protein kinase domain, CSNK1A1L phosphorylates a large number of proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of casein kinase Iα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also recommended for detection of CSNK1A1L of human origin also reactive with additional species, including equine, canine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 281-337 mapping at the C-terminus of casein kinase I&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 281-337 mapping at the C-terminus of casein kinase Iα of human origin |
| Entrez Gene | 1452 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of casein kinase Iα expression in HeLa whole cell lysate.
Application
Western blot analysis of casein kinase Iα expression in K-562 whole cell lysate.
Application
Western blot analysis of CSNK1A1L expression in non-transfected: (A) and human CSNK1A1L transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of casein kinase Iα expression in non-transfected: (A) and mouse casein kinase Iα transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of casein kinase Iα expression in non-transfected 293T: (A), mouse casein kinase Iα transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Application Notes | recommended for detection of casein kinase Iα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also recommended for detection of CSNK1A1L of human origin also reactive with additional species, including equine, canine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
