Application
Western blot analysis of FOXD3 expression in Hep G2 nuclear extract (A) and F9 (B), HL-60 (C) and Jurkat (D) whole cell lysates.Application
Western blot analysis of FOXD3 expression in Hep G2 whole cell lysate.| Product Name | FOXD3 Antibody (F-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of FOXD3 of mouse and human origin by WB, IP and ELISA |
| Modification Notes | Embryonic stem cells require the forkhead transcriptional regulator FoxD3 for survival. Following gastrulation, FoxD3 generally gets downregulated, except in the neural crest. A variety of growth factors induce FoxD3 expression, including FGF8 and SNAIL, maintaining the effected cells in an undifferentiated state. Thus defects in FoxD3 induction may cause premature differentiation and/or migration-asociated birth defects. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of FOXD3 of mouse and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of FOXD3 of mouse and human origin by WB, IP and ELISA |
| Entrez Gene | 27022 |
|---|
Application
Western blot analysis of FOXD3 expression in Hep G2 nuclear extract (A) and F9 (B), HL-60 (C) and Jurkat (D) whole cell lysates.
Application
Western blot analysis of FOXD3 expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
