Application
Western blot analysis of ECHS1 expression in Jurkat whole cell lysate.| Product Name | ECHS1 Antibody (T-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of ECHS1 of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Modification Notes | ECHS1 (enoyl-CoA hydratase 1), also known as SCEH (short chain enoyl-CoA hydratase), is a 290 amino acid protein that localizes to the mitochondrial matrix and belongs to the enoyl-CoA hydratase family. Expressed in muscle, liver and fibroblasts, with low expression in kidney and spleen, ECHS1 exists as a homohexamer that functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. Specifically, ECHS1 catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs, a reaction that is essential for proper lipid metabolism. Human ECHS1 shares 87% homology with its rat counterpart, suggesting a conserved function between species. Multiple isoforms of ECHS1 exist as a result of alternative splicing events. The gene encoding ECHS1 maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of ECHS1 of mouse rat and human origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of ECHS1 of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 1892 |
|---|
Application
Western blot analysis of ECHS1 expression in Jurkat whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
