Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing cytoplasmic localization.Application
Western blot analysis of ADH4 expression in human fetal liver tissue extract.| Product Name | ADH4 Antibody (E-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of ADH4 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | ADH4 (alcohol dehydrogenase 4) is a 380 amino acid protein that belongs to the zinc-containing alcohol dehydrogenase family of enzymes that function to metabolize a wide variety of substrates, including retinol, hydroxysteroids, ethanol, aliphatic alcohols and lipid peroxidation products. Localized to the cytoplasm and existing as a homodimer, ADH4 uses zinc as a cofactor to catalyze the NAD+-dependent conversion of an alcohol to an aldehyde or a ketone, thereby participating in the metabolic degradation of alcohols within the body. Multiple isoforms of ADH4 exist due to alternative splicing events. The gene encoding ADH4 maps to a cluster of alcohol dehydrogenase genes on human chromosome 4, a chromosome that encodes nearly 6% of the human genome and has the largest gene deserts (regions of the genome with no protein encoding genes) of all of the human chromosomes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of ADH4 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of ADH4 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 127 |
|---|
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing cytoplasmic localization.
Application
Western blot analysis of ADH4 expression in human fetal liver tissue extract.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
