Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of C3 expression in non-transfected 293T: (A), human C3 transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Western blot analysis of C3 expression in Hep G2 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing cytoplasmic localization.Application
Western blot analysis of 5 ng human recombinant C3a fusion protein (A) and 100 ng human recombinant C3a (B).| Product Name | C3 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | corresponding to amino acids 541-840 mapping within an internal region of C3 precursor of human origin |
| Modification Notes | Complement C3 precursor contains complement C3 beta chain, complement C3 alpha chain, C3a anaphylatoxin, complement C3b alpha chain, complement C3c fragment, complement C3dg fragment, complement C3g fragment, complement C3d fragment and complement C3f fragment. C3a, C4a, and C5a are potent anaphylatoxins that are released during complement activation, a system of ligand-surface protein interactions specific to cells of hematopoietic lineage that aids in the elimination of pathogens. C3a and C5a secretion correlates with pathophysiological phenotypes such as asthma and bacterial meningitis. Binding of these proteins to their respective G protein-coupled receptors (C3aR, C5aR), which are present on the surface of myeloid leukocytes, induces proinflammatory events such as cellular degranulation, smooth muscle contraction, arachidonic acid metabolism, cytokine release, leukocyte activation and cellular chemotaxis. C3aR is expressed in brain and activated B-lymphocytes whereas C5aR is prevalent on the surface of hepatocyte, lung, smooth muscle, and endothelial cells. Upon activation, C3aR and C5aR are susceptible to rapid GRK-mediated phosphorylation and clathrin-coated vesicle targeting. C5aR utilizes the Ras-Raf-ERK1/2 cascade and couples to Gi/G16 proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of C3 precursor, C3a anaphylatoxin, C3 α, C3 β and C3b α of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | corresponding to amino acids 541-840 mapping within an internal region of C3 precursor of human origin |
|---|---|
| Tissue Specificity | corresponding to amino acids 541-840 mapping within an internal region of C3 precursor of human origin |
| Entrez Gene | 718 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of C3 expression in non-transfected 293T: (A), human C3 transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Western blot analysis of C3 expression in Hep G2 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing cytoplasmic localization.
Application
Western blot analysis of 5 ng human recombinant C3a fusion protein (A) and 100 ng human recombinant C3a (B).| Application Notes | recommended for detection of C3 precursor, C3a anaphylatoxin, C3 α, C3 β and C3b α of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
