Application
Western blot analysis of nucleobindin 2 expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human intestine tissue showing cytoplasmic localization.| Product Name | nucleobindin 2 Antibody (U-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of nucleobindin 2 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Nucleobindin 2, also designated NUCB2 or NEFA, is a 420 amino acid protein that is predominantly expressed in spleen, testis and stomach. It localizes to the Golgi and cisternae of the endoplasmic reticulum (ER) and in the nuclear envelope of neurons in the brain. Nucleobindin 2 contains leucine-zipper and EF-hand motifs, two helix-loop-helix regions, and both a basic and an acidic amino acid region. The leucine zipper structure and the basic amino acid-rich region are responsible for DNA binding. It is a highly charged protein that binds Ca2+ via its EF-hand domains. Nucleobindin 2 is also expressed in the hypothalamic nuclei in rats, which may indicate a role in appetite control. Conversion of nucleobindin 2 to nesfatin-1 in the brain decreases food intake in rats. Nesfatin-1 is identified as a satiety molecule that is involved in mela-nocortin signaling in the hypothalamus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of nucleobindin 2 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of nucleobindin 2 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 4925 |
|---|
Application
Western blot analysis of nucleobindin 2 expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human intestine tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
