Application
Western blot analysis of Tropomyosin β expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human muscle tissue showing cytoplasmic localization.| Product Name | Tropomyosin β Antibody (J-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Tropomyosin β of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Tropomyosin ∫, also known as TPM2 or TMSB, is a 284 amino acid protein that localizes to both the cytoplasm and the cytoskeleton and belongs to the Tropomyosin family of structural proteins. Existing as a heterodimer with a Tropomyosin å protein, Tropomyosin ∫ functions to bind Actin filaments in muscle and non-muscle cells and, via this binding, plays a central role in the regulation of striated muscle contraction and in the stabilization of cytoskeletal Actin filaments. Tropomyosin ∫ is expressed as multiple alternatively spliced isoforms and is present in primary breast cancer tissues, suggesting a role in tumor formation and metastasis. Defects in the gene encoding Tropomyosin ∫ are the cause of nemaline myopathy type 4 (NEM4) and distal arthrogryposis type 1 (DA1), the former of which is a form of congenital myopathy and the latter of which is a form of inherited multiple congenital contractures. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Tropomyosin &beta of mouse and human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Tropomyosin β of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 7169 |
|---|
Application
Western blot analysis of Tropomyosin β expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human muscle tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
