Application
Western blot analysis of CD154 expression in Jurkat (A) and WEHI-3 (B) whole cell lysates.Application
Western blot analysis of human recombinant CD154 fusion protein.| Product Name | CD154 Antibody (H-215) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 47-261 representing the extracellular domain of CD40L of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | Resting B cells can be activated and clonally expanded into antibody-producing cells in response to a combination of cell contact and soluble signals provided by primed helper T (Th) cells. While cytokines IL-4 and IL-13 alone are inadequate for B cell activation, contact with Th cells seems to be sufficient for delivery of proliferative signals. CD40 and CD154 (also designated CD40L or TRAP) comprise a receptor ligand pair central to the transmission of this signal. CD40 is expressed on the surface of B cells and CD154 is expressed on activated T cells. In the presence of such stimulus, IL-4 and IL-13 are capable of triggering immunoglobulin class switching and secretion of IgE. CD154 is a 261 amino acid protein that is is expressed as a soluble cytokine as well as a homotrimeric type II transmembrane protein. Its expression is tightly regulated, and abnormal levels of CD154 are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Mutations in the gene encoding for CD154 are implicated in hyper-IgM immunodeficiency syndrome type 1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| WB:1:100~1:400 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | equine, bovine and porcine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 47-261 representing the extracellular domain of CD40L of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 47-261 representing the extracellular domain of CD40L of human origin |
| Entrez Gene | 959 |
|---|
Application
Western blot analysis of CD154 expression in Jurkat (A) and WEHI-3 (B) whole cell lysates.
Application
Western blot analysis of human recombinant CD154 fusion protein.| Application Notes | WB:1:100~1:400 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
