Application
Western blot analysis of PIASy expression in non-transfected: (A) and human PIASy transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PIASy expression in non-transfected: (A) and human PIASy transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PIASy expression in non-transfected: (A) and human PIASy transfected: (B) 293T whole cell lysates.| Product Name | PIASy Antibody (H-75) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 61-135 mapping within an internal region of PIASy of human origin |
| Modification Notes | The IL-6-type family of cytokines, which includes IL-6 and a number of similar cytokines and growth factors, plays a significant role in regulating gene activation, proliferation and differentiation (1). Transcription factors of the STAT family are involved in IL-6 family-mediated signal transduction pathways, and upon activation undergo phosphorylation, dimerization and translocation to the nucleus (2,3). The duration and intensity of a cell's response to cytokines can be adjusted by the effect of several regulatory mechanisms (4). One example involves the protein inhibitor of activated signal transducer and activator of transcription (STAT) family (PIAS family) of proteins, which act as negative regulators of STATs in cytokine signaling (5,6). PIAS proteins are able to coactivate steroid receptor-dependent transcription as well (7). Human PIASy is a 510 amino acid transcriptional corepressor of the androgen receptor (AR) (8). In addition, PIASy may regulate p53-mediated events and may direct p53 into a transactivation-independent mode of apoptosis (9). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PIASy of human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 61-135 mapping within an internal region of PIASy of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 61-135 mapping within an internal region of PIASy of human origin |
| Entrez Gene | 51588 |
|---|
Application
Western blot analysis of PIASy expression in non-transfected: (A) and human PIASy transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PIASy expression in non-transfected: (A) and human PIASy transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PIASy expression in non-transfected: (A) and human PIASy transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of PIASy of human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
