| Product Name | FIH-1 Antibody (H-229) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 15-243 mapping within an internal region of FIH-1 of human origin |
| Modification Notes | Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1å. Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the ∫-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1å. This hydroxylation event blocks the association of HIF-1å with co-activators. FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1å. In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm. The structure of FIH-1 includes a jellyroll-like ∫-barrel containing ferrous-binding triad residues. The gene encoding human FIH-1 maps to chromosome 10q24. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of FIH-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 15-243 mapping within an internal region of FIH-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 15-243 mapping within an internal region of FIH-1 of human origin |
| Entrez Gene | 55662 |
|---|
| Application Notes | recommended for detection of FIH-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
