Application
Western blot analysis of Integrin α4 expression in non-transfected 293T: (A), human Integrin α4 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse liver tissue showing membrane localization.Application
Western blot analysis of Integrin α4 expression in MOLT-4 (A) and Jurkat (B) whole cell lysates.| Product Name | Integrin α4 Antibody (H-210) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 796-1005 of Integrin α4 of human origin |
| Modification Notes | Integrins are heterodimers composed of noncovalently associated transmembrane å and ∫ subunits. The 16 å and 8 ∫ subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including fibronectin, collagen and vitronectin. Certain integrins can also bind to soluble ligands such as fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors; both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Integrin α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 796-1005 of Integrin &alpha 4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 796-1005 of Integrin α4 of human origin |
| Entrez Gene | 3676 |
|---|
Application
Western blot analysis of Integrin α4 expression in non-transfected 293T: (A), human Integrin α4 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse liver tissue showing membrane localization.
Application
Western blot analysis of Integrin α4 expression in MOLT-4 (A) and Jurkat (B) whole cell lysates.| Application Notes | recommended for detection of Integrin α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
