Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing mitochondria localization.Application
Western blot analysis of HIGD1A expression in Hep G2 whole cell lysate.| Product Name | HIGD1A Antibody (D-16) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of HIGD1A of human origin |
| Modification Notes | HIGD1A (HIG1 domain family member 1A), also known as HIG1 (hypoxia-inducible gene 1 protein) or HSPC010, is a 93 amino acid multi-pass membrane protein that contains one HIG1 domain. Existing as two alternatively spliced isoforms, the gene encoding HIGD1A maps to human chromosome 3, which contains approximately 214 million bases encoding over 1,100 genes. Notably, there is a chemokine receptor gene cluster and a variety of human cancer related loci on chromosome 3. Particular regions of the chromosome 3 short arm are deleted in many types of cancer cells. Key tumor suppressing genes on chromosome 3 encode apoptosis mediator RASSF1, cell migration regulator HYAL1 and angiogenesis suppressor SEMA3B. Marfan Syndrome, porphyria, von Hippel-Lindau syndrome, osteogenesis imperfecta and Charcot-Marie-Tooth Disease are a few of the numerous genetic diseases associated with chromosome 3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HIGD1A of human origin by WB, IP, IF and ELISA non cross-reactive with HIGD 1B and HIGD 2A: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of HIGD1A of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of HIGD1A of human origin |
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing mitochondria localization.
Application
Western blot analysis of HIGD1A expression in Hep G2 whole cell lysate.| Application Notes | recommended for detection of HIGD1A of human origin by WB, IP, IF and ELISA non cross-reactive with HIGD 1B and HIGD 2A: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
