Application
Western blot analysis of Parafibromin expression in non-transfected 293T: (A), human Parafibromin transfected 293T: (B) and C32 (C) whole cell lysates.Application
Western blot analysis of Parafibromin expression in non-transfected 293T: (A) and mouse Parafibromin transfected 293T: (B) whole cell lysates.Application
Western blot analysis of Parafibromin expression in non-transfected 293T: (A), human Parafibromin transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immuno?fluorescence staining of methanol-fixed HeLa cells showing nuclear local?ization.| Product Name | Parafibromin Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 232-531 mapping at the C-terminus of parafibromin of human origin |
| Modification Notes | Parathyroid tumors are heterogeneous and diagnosis of the disease is often difficult. The Parafibromin protein may be important as a marker for diagnosing parathyroid carcinoma. Parafibromin is encoded by the endocrine tumor suppressor gene CDC73 (cell division cycle 73, Paf1/RNA polymerase II complex component), alternatively known as the HRPT2 (hyperparathyroidism-jaw tumor syndrome 2) gene. The human CDC73 gene, which maps to chromosome 1q25, is the human homolog of Saccharomyces cerevisiae Cdc73 and is responsible for the hyperparathyroidism with jaw tumor syndrome (HPT-JT). Parafibromin is part of the RNA polymerase II/Paf1 complex, which is crucial for histone modification. This Parafibromin complex binds to both the nonphosphorylated forms and the Ser 2 and Ser 5 phosphorylated forms of the RNA polymerase II large subunit. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of parafibromin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 232-531 mapping at the C-terminus of parafibromin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 232-531 mapping at the C-terminus of parafibromin of human origin |
| Entrez Gene | 79577 |
|---|
Application
Western blot analysis of Parafibromin expression in non-transfected 293T: (A), human Parafibromin transfected 293T: (B) and C32 (C) whole cell lysates.
Application
Western blot analysis of Parafibromin expression in non-transfected 293T: (A) and mouse Parafibromin transfected 293T: (B) whole cell lysates.
Application
Western blot analysis of Parafibromin expression in non-transfected 293T: (A), human Parafibromin transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immuno?fluorescence staining of methanol-fixed HeLa cells showing nuclear local?ization.| Application Notes | recommended for detection of parafibromin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
