Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.Application
Western blot analysis of DnaJC28 expression in non-transfected 293T: (A), human DnaJC28 transfected 293T: (B), MES-SA/Dx5 (C) and Hep G2 (D) whole cell lysates and mouse brain tissue extract (E).Application
Western blot analysis of DnaJC28 expression in MES-SA/Dx5 (A) and Hep G2 (B) whole cell lysates and mouse spleen (C), mouse brain (D), mouse testis (E) and mouse embryo (F) tissue extracts.| Product Name | DnaJC28 Antibody (S-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of DnaJC28 of human origin |
| Modification Notes | The smallest of the human chromosomes, 21 makes up about 1.5% of the human genome. Chromosome 21 contains nearly 300 genes and 47 million base pairs. Down syndrome, also known as trisomy 21, is the disease most commonly associated with chromosome 21. Alzheimer's disease, Jervell and Lange-Nielsen syndrome and amyotrophic lateral sclerosis are also associated with chromosome 21. Translocations are found to occur between chromosome 21 and 8, and chromosome 21 and 12, in certain leukemias. DnaJC28 (DnaJ homolog subfamily C member 28), also known as C21orf55, C21orf78 or MGC27620 is a 388 amino acid protein that is expressed in the fetal and adult brain, testis, uterus, spleen and liver. It has been suggested that DnaJC28 may play a role in protein folding or as a chaperone. The DnaJC28 gene product has been provisionally designated DnaJC28 pending further characterization. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DnaJC28 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of DnaJC28 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of DnaJC28 of human origin |
| Entrez Gene | 54943 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.
Application
Western blot analysis of DnaJC28 expression in non-transfected 293T: (A), human DnaJC28 transfected 293T: (B), MES-SA/Dx5 (C) and Hep G2 (D) whole cell lysates and mouse brain tissue extract (E).
Application
Western blot analysis of DnaJC28 expression in MES-SA/Dx5 (A) and Hep G2 (B) whole cell lysates and mouse spleen (C), mouse brain (D), mouse testis (E) and mouse embryo (F) tissue extracts.| Application Notes | recommended for detection of DnaJC28 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
