Application
Western blot analysis of Urm1 expression in 293 showing nontransfected (A) and transfected (B) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung carcinoma tissue showing cytoplasmic staining.| Product Name | Urm1 Antibody (N-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Urm1 of human origin by WB, IP, IF and IHC(P) |
| Modification Notes | Ubiquitin (Ub) is among the most phylogenetically conserved proteins known. The primary function of this small, 76 amino acid protein is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome. Many ubiquitin-like proteins function as post-translational protein modifiers, such as members of the SUMO protein family, however some ubiquitin-like proteins regulate protein-protein interactions and cell cycle events, thereby functioning outside of the traditional ubiquitination pathway. Urm1 (Ubiquitin-related modifier 1 homolog) is a 101 amino acid protein that primarily functions in the post-translational modification of proteins by way of the urmylation pathway. In studies with Saccharomyces cerevisiae, it has been found that Urm1 covalently binds to its E1 activating enzyme, Uba4p, to conjugate alkyl hydroperoxide reductase (Ahp1). It is hypothesized that this complex may then play a role in the oxidative-stress response in mammals. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Urm1 of human origin by WB IP IF and IHC(P) |
|---|---|
| Tissue Specificity | recommended for detection of Urm1 of human origin by WB, IP, IF and IHC(P) |
| Entrez Gene | 81605 |
|---|
Application
Western blot analysis of Urm1 expression in 293 showing nontransfected (A) and transfected (B) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung carcinoma tissue showing cytoplasmic staining.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
